Research Groups in Danmark

Martin Dufva

Assoc. Prof. & Groupleader of Fluidic Array Systems and Technology, DTU Health Tech, Technical University of Danmark


Keywords describing the research

  • High throughput couples organ systems

  • 3D printing

  • intestine modelling

  • fluidics and dynamics

  • gelatin hydrogels

Recent publications

  • Tissue Engineering: 3D Printed Stackable Titer Plate Inserts Supporting Three Interconnected Tissue Models for Drug Transport Studies. (Adv. Biosys). 7/2020). Morten Leth Jepsen  Andreas Willumsen  Chiara Mazzoni  Anja Boisen  Line Hagner Nielsen  Martin Dufva. 2020, Volume 4, Issue 7, pp. 1900289. https://doi.org/10.1002/adbi.201900289

  • Jepsen ML, Nielsen LH, Boisen A, Almdal K, Dufva M. Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering. Biopolymers. 2019;110(1):e23241. doi:10.1002/bip.23241. https://doi.org/10.1002/bip.23241

  • Tan H-Y, Trier S, Rahbek UL, Dufva M, Kutter JP, Andresen T A multi-chamber microfluidic intestinal barrier model using Caco-2 cells for drug transport studies. PLoS. 2018;13(5):e0197101. https://doi.org/10.1371/journal.pone.0197101

  • Starokozhko V, Hemmingsen M, Larsen L, et al. Differentiation of human-induced pluripotent stem cell under flow conditions to mature hepatocytes for liver tissue engineering. J Tissue Eng Regen Med. 2018


  • Pimentel C R, Ko SK, Caviglia C, Wolff A, Emnéus J, Keller SS, Dufva M.  Three-dimensional fabrication of thick and densely populated soft constructs with complex and actively perfused channel network. 2018. Acta Biomaterialia, 65, 174-184. https://doi.org/10.1016/j.actbio.2017.10.047

    ;12(5):1273-1284. https://doi.org/10.1002/term.2659

Johan Ulrik Lind

PI of Tailored Materials and Tissues Group, Technical University of Denmark 


Keywords describing the research

  • Bio-Functional materials

  • Additive manufacturing

  • Instrumented Microphysiological Systems

  • Tissue engineering

  • Bio-printing

Recent publications

  • Endothelial extracellular vesicles contain protective proteins and rescue ischemia-reperfusion injury in a human heart-on-chip, Science Translational Medicine, 2020, 12 (565), Yadid, J.U. Lind, H.A.M. Ardoña , et al.

  • 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices, J. Kajtez, S. Buchmann, S. Vasudevan, M. Birtele, S. Rocchetti, C.J. Pless, A. Heiskanen, R.A. Barker, A. Martínez-Serrano, M. Parmar, J.U. Lind, J. Emnéus, Advanced Science, 2020, 7, 2001150

  • Large-scale spontaneous self-organization and maturation of skeletal muscle tissues on ultra-compliant gelatin hydrogel substrates, J.H. Jensen, S.D. Cakal, J. Li, C.J. Pless, C. Radeke, M.L. Jepsen, T.E. Jensen, M. Dufva J.U. Lind, Scientific Reports, 2020, 10, 13305

  • Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing, J.U. Lind, T.A. Busbee, A.D. Valentine, et al., Nature Materials, 2017, 16, pp: 303-308

  • Cardiac microphysiological devices with flexible thin-film sensors for higher-throughput drug screening, J.U. Lind, M. Yadid, I. Perkins, et al., Lab on a Chip, 2017, 17, pp: 3692-3703

Jenny Emnéus

Prof. at Dept. Biotechnology and Bioengineering, Technical University of Danmark


Keywords describing the research

  • brain organoids

  • conductive scaffolds

  • bioelectrochemistry

  • optogenetics

  • drug delivery

Recent publications

  • Next generation human brain models: Engineered flat brain organoids featuring gyrification; Authors: Theresa S.P. Rothenbücher, Hakan Gürbüz, Marta P Pereira, Arto Heiskanen, Jenny Emneus, Alberto Martinez-Serrano. Biofabrication 13 (2021) 011001.
  • On-demand reversible UV-triggered interpenetrating polymer network based drug delivery system using the spiropyran-merocyanine hydrophobicity switch. Mozhdeh Ghani, Arto Heiskanen, Janko Kajtez, Babak Rezaei, Niels Bent Larsen, Peter Thomsen, Anders Kristensen, Airidas Žukauskas, Martin Alm, Jenny Emnéus. ACS Applied Materials and Interfaces 2021, 13, 3, 3591–3604.
  • 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices. Kajtez, Janko; Buchmann, Sebastian; Vasudevan, Shashank; Birtele, Marcella; Rocchetti, Stefano; Pless, Christian Jonathan; Heiskanen, Arto; Barker, Roger A; Martinez-Serrano, Alberto; Parmar, Malin; Lind, Johan Ulrik; Emnéus, Jenny. Advanced Science, vol 7, issue 16, 2020, article number: Cover Page.
  • Pyrolytic carbon nanograss enhances neurogenesis and dopaminergic differentiation of human midbrain neural stem cells. Asif, S. García-Lopéz, A. Heiskanen, A. Martínez-Serrano, S.S. Keller, M. Pereira, J. Emnéus, Advanced Healthcare Materials (2020) 2001108
  • Leaky Optoelectrical Fiber for Optogenetic Stimulation and Electrochemical Detection of Dopamine Exocytosis from Human Dopaminergic Neurons. S. Vasudevan, J. Kaltez. A.-I. Bunea, A. Gonzalez-Ramos, T. Ramos-Moreno, A. Heiskanen, M. Kokaia, N. B. Larsen, A. Martinez-Serrano, S. S. Keller, and J. Emnéus. Advanced Sciences, vol 6, issue 24, article number:1902011,

    Research Groups in Finland

    Katriina Aalto-Setälä

    PI of Heart group, Tampere University


    Keywords describing the research

    • iPSC

    • cardiomyocytes

    • hepatocytes

    • disease modeling

    • Organ-on-Chip

    Recent publications

    • Viiri LE, Rantapero T, Kiamehr M, Viiri K, Niskanen H, Nykter M, Kaikkonen MU, Aalto-Setälä K. GRO-sequencing identifies transcriptional regulators and ncRNAs important for iPSC to hepatocyte differentiation. Scientific reports 2019 Mar 5;9 (1) 3562 IF 4.12

    • Juhola M, Penttinen K, Joutsijoki H, Aalto-Setälä K. Analysis of Drug effectys on iPSC cardiomyocytes with machine learning. Ann Biomed Eng. 2020 May 4. doi: 10.1007/s10439-020-02521-0. Online ahead of print.PMID: 32367466

    • Shah D, Prajapati C, Penttinen K, Maria Cherian R, Koivumäki JT, Alexanova A, Hyttinen J, Aalto-Setälä K. hiPSC-derived cardiomyocyte model of LQT2 syndrome derived from asymptomatic and symptomatic mutation carriers reproduces clinical differences in aggregates but not in single cells. Cells. 2020 May 7;9(5):E1153. doi: 10.3390/cells9051153.PMID: 32392813

    • Häkli MIE, Kerutzer JM, Mäki A, Välimäki H, Lappi H, Huhtala A, Kallio P, Aalto-Setälä K, Pekkanen-Mattila M. Human induced pluripotent stem cell -based system for modelling cardiac ischemia. Sci Rep. 2021 Feb 18;11(1):4153. doi: 10.1038/s41598-021-83740-w.PMID: 33603154

    • Prajapati C,  Ojala M,  Lappi H,  Aalto-Setälä K,  Pekkanen-Mattila M.  Electrophysiological Evaluation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Obtained by Different Methods.  Stem Cell Res. 2021 Mar;51:102176. doi: 10.1016/j.scr.2021.102176. Epub 2021 Jan 15.PMID: 33485184


    Pasi Kallio
    Prof. & PI of Micro- and Nanosystems Research Group, Tampere University


    Keywords describing the research

    • microfluidics
    • microsensors and image-based measurements
    • microrobotics
    • automation and control of systems

    Recent publications

    • Ristola, M., Fedele, C., Hagman, S., Sukki, L., Kapucu, E.F., Mzezeva, R., Hyvärinen, T., Kallio, P., Priimagi, A. & Narkilahti, S. “Directional Growth of Human Neuronal Axons in a Microfluidic Device with Nanotopography on Azobenzene-Based Material”. Accepted for publication in Advanced Materials Interfaces.
    • Häkli, M., Kreutzer, J., Mäki, A.J., Välimäki, H., Lappi, H., Huhtala, H., Kallio, P., Aalto-Setälä, K. & Pekkanen-Mattila, M. ”Human Induced Pluripotent Stem Cell-based Platform for Modeling Cardiac Ischemia”. Accepted for publication in Scientific Reports.
    • Pelkonen, A., Mzezewa, R., Sukki, L., Ryynänen, T., Kreutzer, J., Hyvärinen, T., Vinogradov, A., Aarnos, L., Lekkala, J., Kallio, P. & Narkilahti, S. “A modular Brain-on-a-chip for Modelling Epileptic Seizures with Functionally Connected Human Neuronal Networks” Biosensors and Bioelectronics, Volume 168, November 2020, 112553, doi:10.1016/j.bios.2020.112553
    • Kattipparambil Rajan, D., Mäki, A.-J., Pekkanen-Mattila, M., Kreutzer, J., Ryynänen, T., Välimäki, H., Verho, J., Koivumäki, J., Ihalainen, H., Aalto-Setälä, K., Kallio, P. & Lekkala, J. ”Cardiomyocytes: Analysis of Temperature Response and Signal Propagation between Dissociated Clusters using Novel Video-based Movement Analysis Software”. IEEE Access. Volume 8, June 2020, pp. 109275 – 109288, DOI: 10.1109/ACCESS.2020.3001191
    • Välimäki, H., Hyvärinen, T., Leivo, J., Iftikhar, H., Pekkanen-Mattila, M., Kattipparambil Rajan, D., Verho, J., Kreutzer, J., Ryynänen, T., Pirhonen, J., Aalto-Setälä, K., Kallio, P., Narkilahti, S. & Lekkala, J. ”Covalent Immobilization of Luminescent Oxygen Indicators Reduces Cytotoxicity”. Biomedical Microdevices, Vol 22, Article number: 41, June 2020, https://doi.org/10.1007/s10544-020-00495-3

    Susanna Narkilahti
    PhD, Adj. Prof and Leader of NeuroGroup, Tampere University


    Keywords describing the research

    • organ-on-chip

    • in vitro models

    • disease modeling

    • micro-electrode arrays

    • neurons

    • astrocytes, microglia

    Recent publications

    • Ristola M, Fedele C, Hagman S, Sukki L, Kapucu F.E, Mzezeva R, Hyvärinen T, Kallio P, Priimagi A, Narkilahti S (2021) Directional Growth of Human Neuronal Axons in a Microfluidic Device with Nanotopography on Azobenzene-Based Material, 03 May 2021, Advanced Materials Interfaces. https://doi.org/10.1002/admi.202100048
    • Honkamäki L, Joki T, Grigoryev NA, Levon K, Ylä-Outinen L, Narkilahti S (2020) Novel method to produce a layered 3D scaffold for human pluripotent stem cell-derived neuronal cells. J Neurosci Methods. 2020 Dec 17;350:109043. DOI: 10.1016/j.jneumeth.2020.109043
    • Juntunen M, Hagman S, Moisan A, Narkilahti S, Miettinen S (2020) In vitro oxygen-glucose deprivation-induced stroke models with human neuroblastoma cell- and induced pluripotent stem cell-derived neurons. Stem Cell International Vol 2020, Article ID 8841026, 13 pages, DOI: 10.1155/2020/8841026.
    • Pelkonen A, Mzezewa R, Sukki L, Ryynänen T, Kreutzer J, Hyvärinen T, Vinogradov A, Aarnos L, Lekkala J, Kallio P, Narkilahti S (2020) A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks. Biosensors and Bioelectronics Volume 168, 15 November 2020. DOI: 10.1016/j.bios.2020.112553.
    • Hyvärinen T, Hyysalo A, Kapucu FE, Aarnos L, Vinogradov A, Eglen S.J, Ylä-Outinen L, Narkilahti S (2019) Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures. Sci Rep. 2019 Nov 20;9(1):17125. DOI: 10.1038/s41598-019-53647-8

    Cecilia Sahlgren

    Prof. & PI of The Cell Fate Lab, Åbo Akademi University


    Keywords describing the research

    • cell fate

    • mechanobiology

    • cell organization and  tissue remodeling

    • disease modeling

    • tissue engineering

    Recent publications

    • Ristori T, Stassen OMJA, Sahlgren CM, Loerakker S. Lateral Induction Limits the Impact of Cell Connectivity on Notch Signaling in Arterial Walls. Int J Numer Method Biomed Eng. 2020. https://doi.org/10.1002/cnm.3323
    • van Engeland NCA#, Suarez Rodriguez F#, Rivero-Müller A, Ristori T, Duran CL, Stassen OMJA, Antfolk D, Driessen RCH, Ruohonen S, Ruohonen ST, Nuutinen S, Savontaus E, Loerakker S, Bayless KJ, Sjöqvist M, Bouten CVC, Eriksson JE, Sahlgren CM. Vimentin regulates Notch signaling strength and arterial remodeling in response to hemodynamic stress. Sci Rep. 2019. https://doi.org/10.1038/s41598-019-48218-w 
    • Driessen RCH, Stassen OMJA, Sjöqvist M, Suarez Rodriguez F, Grolleman J, Bouten CVC, Sahlgren CM. Shear stress induces expression, intracellular reorganization and enhanced Notch activation potential of Jagged1. Integr Biol (Camb). 2018. https://doi.org/10.1039/c8ib00036k

    • van Engeland NCA, Pollet AMAO, den Toonder JMJ, Bouten CVC, Stassen OMJA, Sahlgren CM. A biomimetic microfluidic model to study signalling between endothelial and vascular smooth muscle cells under hemodynamic conditions. Lab Chip. 2018. https://doi.org/10.1039/c8lc00286j
    • Loerakker S*, Stassen OMJA, Ter Huurne FM, Boareto M, Bouten CVC, Sahlgren CM*. Mechanosensitivity of Jagged-Notch signaling can induce a switch-type behavior in vascular homeostasis. Proc Natl Acad Sci U S A. 2018. https://doi.org/10.1073/pnas.1715277115 

    Jari Hyttinen

    Prof. & PI of Computational Biophysics and Imaging group, Tampere University


    Keywords describing the research

    • quantitative bioimaging

    • in-silico modelling

    • bioelectronics

    • biophysics

    • bioimpedance

    Recent publications

    • Tamminen I, Lehto K, Hannula M, Ojansivu M, Johansson L, Kellomäki M, Miettinen S, Aula A, Ihalainen T, Hyttinen J. A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samples. Commun Biol. 2020 Oct 2;3(1):548. doi: 10.1038/s42003-020-01273-w. PMID: 33009501; PMCID: PMC7532209.

    • Belay B, Koivisto JT, Parraga J, Koskela O, Montonen T, Kellomäki M, Figueiras E, Hyttinen J. Optical projection tomography as a quantitative tool for analysis of cell morphology and density in 3D hydrogels. Sci Rep. 2021 Mar 22;11(1):6538. doi: 10.1038/s41598-021-85996-8. PMID: 33753803; PMCID: PMC7985381.

    • Lenk K, Satuvuori E, Lallouette J, Ladrón-de-Guevara A, Berry H, Hyttinen JAK. A Computational Model of Interactions Between Neuronal and Astrocytic Networks: The Role of Astrocytes in the Stability of the Neuronal Firing Rate. Front Comput Neurosci. 2020 Jan 22;13:92. doi: 10.3389/fncom.2019.00092. PMID: 32038210; PMCID: PMC6987305.

    • Paci M, Passini E, Klimas A, Severi S, Hyttinen J, Rodriguez B, Entcheva E. All-Optical Electrophysiology Refines Populations of In Silico Human iPSC-CMs for Drug Evaluation. Biophys J. 2020 May 19;118(10):2596-2611. doi: 10.1016/j.bpj.2020.03.018. Epub 2020 Apr 4. PMID: 32298635; PMCID: PMC7231889.

    • Subramaniyam, N. P., Donner, R. V., Caron, D., Panuccio, G., & Hyttinen, J. (2021). Causal coupling inference from multivariate time series based on ordinal pattern transition networks. arXiv preprint arXiv:2010.00948. (accepted to be published in Nonlinear Dynamics 2021)

    Susanna Miettinen
    Prof. & PI of Adult Stem Cell Group, Tampere University


    Keywords describing the research

    • mesenchymal stem/stromal cells
    • tissue engineering
    • adipose tissue
    • bone
    • vasculature

    Recent publications

    • Rangasami, VK, Nawale, G, Asawa, K, Kadekar, S, Samanta, S, Nilsson, B et al.. Pluronic Micelle-Mediated Tissue Factor Silencing Enhances Hemocompatibility, Stemness, Differentiation Potential, and Paracrine Signaling of Mesenchymal Stem Cells. Biomacromolecules. 2021;22 (5):1980-1989. doi: 10.1021/acs.biomac.1c00070. PubMed PMID:33813822 PubMed Central PMC8154246.
    • Bakreen, A, Juntunen, M, Dunlop, Y, Ugidos, IF, Malm, T, Miettinen, S et al.. Additive Behavioral Improvement after Combined Cell Therapy and Rehabilitation Despite Long-Term Microglia Presence in Stroke Rats. Int J Mol Sci. 2021;22 (4):. doi: 10.3390/ijms22041512. PubMed PMID:33546370 PubMed Central PMC7913568

    • Juntunen, M, Hagman, S, Moisan, A, Narkilahti, S, Miettinen, S. In Vitro Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons. Stem Cells Int. 2020;2020 :8841026. doi: 10.1155/2020/8841026. PubMed PMID:33178286 PubMed Central PMC7647751.

    • Hyväri, L, Vanhatupa, S, Halonen, HT, Kääriäinen, M, Miettinen, S. Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells. Stem Cells Int. 2020;2020 :8853541. doi: 10.1155/2020/8853541. PubMed PMID:33029150 PubMed Central PMC7527895.
    • Tamminen, I, Lehto, K, Hannula, M, Ojansivu, M, Johansson, L, Kellomäki, M et al.. A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samples. Commun Biol. 2020;3 (1):548. doi: 10.1038/s42003-020-01273-w. PubMed PMID:33009501 PubMed Central PMC7532209.

      Tiina Sikanen

      Assoc. Prof. at Drug Research Program, Faculty of Pharmacy, University of Helsinki


      Keywords describing the research

      • Drug metabolism

      • drug-drug interactions

      • liver-on-a-chips

      • immobilized enzyme reactors

      • digital microfluidics

      • polymer microfabrication

      Recent publications

      • The material-enabled oxygen control in thiol-ene microfluidic channels and its feasibility for subcellular drug metabolism assays under hypoxia in vitro, Lab Chip, 2021, 21, 1820-1831.
        Kiiski, I., Järvinen, P., Ollikainen, E., Jokinen, V., Sikanen, T.M.
      • Simultaneous Culturing of Cell Monolayers and Spheroids on a Single Microfluidic Device for Bridging the Gap between 2D and 3D Cell Assays in Drug Research, Adv. Funct. Mater. 2020, 2000479 (11 pp).
        Järvinen, P., Bonabi, A., Jokinen, V., Sikanen, T.M.
      • Cell Proliferation on Common 3D Printing Materials Used in Stereolithography of Microfluidic Devices, Lab Chip 2020, 20, 2372 – 2382.
        Piironen, K., Haapala, M., Talman, V., Järvinen, P., Sikanen, T.M.
      • Digital microfluidics-enabled analysis of individual variation in liver cytochrome P450 activity”, Anal. Chem., 2020, 92, 14693–14701.
        Sathyanarayanan, G., Haapala, M., Sikanen, T.M.
      • Overcoming the Pitfalls of Cytochrome P450 Immobilization Through the Use of Fusogenic Liposomes, Adv. Biosyst. 2019, 3, 3, 1800245 (6 pp).
        Kiiski, I., Pihlaja, T., Urvas, L., Witos, J., Wiedmer, S.K., Jokinen, V.P., Sikanen, T.M.

      Research Groups in Iceland

      Thorarinn Gudjonsson
      Prof. & Dean of the Faculty of Medicine, University of Iceland


      Keywords describing the research

      • Breast

      • Lung

      • Morphogenesis

      • stem cells

      • drug screening

      • 3D cell culture

      Recent publications

      • Briem E, Budkova Z, Sigurdardottir AK, Hilmarsdottir B, Kricker J, Timp W, Magnusson MK, Traustadottir GA, Gudjonsson T. MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin. Mech Dev. 2019 Feb;155:34-47. 

      • Arason AJ, Joelsson JP, Valdimarsdottir B, Sigurdsson S, Gudjonsson A, Halldorsson S, Johannsson F, Rolfsson O, Lehmann F, Ingthorsson S, Cherek P, Gudmundsson GH,

        Gardarsson FR, Page CP, Baldursson O, Gudjonsson T, Kricker JA. Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia. Respir Res. 2019 Jun 24;20(1):129.

      • Morera E, Steinhäuser SS, Budkova Z, Ingthorsson S, Kricker J, Krueger A, Traustadottir GA, Gudjonsson T. YKL-40/CHI3L1 facilitates migration and invasion in HER2

        overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation. In Vitro Cell Dev Biol Anim. 2019 Dec;55(10):838-853.

      • Steinhaeuser SS, Morera E, Budkova Z, Schepsky A, Wang Q, Rolfsson O, Riedel A, Krueger A, Hilmarsdottir B, Maelandsmo GM, Valdimarsdottir B, Sigurdardottir AK,

        Agnarsson BA, Jonasson JG, Ingthorsson S, Traustadottir GA, Oskarsson T, Gudjonsson T. ECM1 secreted by HER2-overexpressing breast cancer cells promotes formation of a vascular niche accelerating cancer cell migration and invasion. Lab Invest. 2020 Jul;100(7):928-944.

      • Schepsky, A., G. A. Traustadottir, J. P. Joelsson, S. Ingthorsson, J. Kricker, J. T. Bergthorsson, A. Asbjarnarson, T. Gudjonsson, N. Nupponen, A. Slipicevic, F. Lehmann and T. Gudjonsson (2020). Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines. Cancer Med 9(18): 6726-6738.

      Bylgja Hilmarsdóttir

      PhD & Research scientist at Molecular pathology department, Landspitali University Hospital

      and at Biomedical Center, University of Iceland


      Keywords describing the research

      • Organoids
      • drug resistance
      • microenvironment
      • pancreatic cancer
      • breast cancer

      Recent publications

      • Budkova, Z., Sigurdardottir, A.K., Briem, E., Bergthorsson, J.T., Sigurdsson, S., Magnusson, M.K., Traustadottir, G.A., Gudjonsson, T., and Hilmarsdottir, B. (2020). Expression of ncRNAs on the DLK1-DIO3 Locus Is Associated With Basal and Mesenchymal Phenotype in Breast Epithelial Progenitor Cells. Front Cell Dev Biol 8, 461.

      • Arason, A., Agnarsson, B.A., Johannesdottir, G., Johannsson, O.T., Hilmarsdottir, B., Reynisdottir, I., and Barkardottir, R.B. (2019). The BRCA1 c.4096+3A>G Variant Displays Classical Characteristics of Pathogenic BRCA1 Mutations in Hereditary Breast and Ovarian Cancers, But Still Allows Homozygous Viability. Genes (Basel) 10.

      • Grinde, M.T., Hilmarsdottir, B., Tunset, H.M., Henriksen, I.M., Kim, J., Haugen, M.H., Rye, M.B., Mælandsmo, G.M., and Moestue, S.A. (2019). Glutamine to proline conversion is associated with response to glutaminase inhibition in breast cancer. Breast Cancer Res. 21, 61.

      • Briem, E., Ingthorsson, S., Traustadottir, G.A., Hilmarsdottir, B., and Gudjonsson, T. (2019). Application of the D492 Cell Lines to Explore Breast Morphogenesis, EMT and Cancer Progression in 3D Culture. J Mammary Gland Biol Neoplasia.

      • Hilmarsdottir, B., Briem, E., Halldorsson, S., Kricker, J., Ingthorsson, S., Gustafsdottir, S., Mælandsmo, G.M., Magnusson, M.K., and Gudjonsson, T. (2017). Inhibition of PTP1B disrupts cell-cell adhesion and induces anoikis in breast epithelial cells. Cell Death Dis 8, e2769.

      Research Groups in Norway

      photographer: Malin Arnesson

      Stefan Krauss

      Prof. & Director of Hybrid Technology Hub - CoE, University of Oslo


      Keywords describing the research

      • Developmental biology and morphogenetic signaling (HH/WNT)

      • Drug development on morphogenetic signals

      • Organ-on-a-chip platform development

      • Liver organoids Gastruloids 

      Recent publications

      • Thermoplastic elastomer (TPE) – Poly-(methyl methacrylate) (PMMA) hybrid devices for active pumping PDMS-free Organ-on-a-Chip systems. Biosensors 2021, 11(5), 162. Mathias Busek, Steffen Nøvik, Aleksandra Aizenshtadt, Mikel Amirola-Martinez, Thomas Combriat, Stefan Grünzner, Stefan Krauss

      • Pre-clinical lead optimization of a 1,2,4-triazole based tankyrase inhibitor. J Med Chem. 2020, Jul 9;63(13):6834-6846. Jo Waaler, Ruben G. G. Leenders, Sven T. Sowa, Shoshy Alam Brinch, Max Lycke, Piotr Nieczypor, Sjoerd Aertssen, Sudarshan Murthy, Albert Galera-Prat, Eddy Damen, Anita Wegert, Marc Nazaré, Lari Lehtiö, and Stefan Krauss

      • 3D cell culture models and organ‐on‐a‐chip: Meet separation science and mass spectrometry. Electrophoresis 2020, 41 (1-2), 56-64. Ann Lin, Frøydis Sved Skottvoll, Simon Rayner, Stig Pedersen‐Bjergaard, Gareth Sullivan, Stefan Krauss, Steven Ray Wilson, Sean Harrison

      • Pancreas-on-a-chip technology for transplantation applications. Current Diabetes Reports 2020, 20 (12), 1-13. Shadab Abadpour; Aleksandra Aizenshtadt; Petter Angell Olsen; Kayoko Shoji; Steven Ray Wilson; Stefan Krauss; Hanne Scholz

      • The tankyrase inhibitor G007-LK inhibits small intestine Lgr5+ stem cell proliferation without altering tissue morphology. Biol Res. 2018, Jan 9;51(1):3. Jens Henrik Norum, Ellen Skarpen, Andreas Brech, Raoul Kuiper, Wanja Kildal, Jo Waaler, Stefan Krauss and Therese Sørlie

      Bjørn Stokke

      Prof. at Dept. of Physics,

      The Norwegian University of Science and Technology, Trondheim


      Keywords describing the research

      • Biopolymers

      • Single-molecule

      • Hydrogels

      • Biosensing

      • Microfluidics


      Recent publications

      • Håti, A.G., Basset, D., Ribe, J.M., Sikorski, P., Weitz, D.A., and Stokke, B.T. Versatile, cell and chip friendly method to gel alginate in microfluidic devices. Lab on a Chip 16 (2016) 3718-3727.

      • Jonasova, E.P., Bjørkøy, A., and Stokke, B.T., Toehold length of target ssDNA affects its reaction-diffusion behaviour in DNA-responsive DNA-co-acrylamide hydrogels; Biomacromolecules, 21 (2020) 1687-1699

      • Øvreeide, I.H., Zoellner, A., Mielnik, M.M. and Stokke, B.T. Curved passive mixing structures: A robust design to obtain efficient mixing and mass transfer in microfluidic channels. J. Micromech. Microeng. 31 (2021) 015006

      • Ahmed, H., and Stokke, B.T., Fabrication of monodisperse alginate microgel beads by microfluidic picoinjection: A chelate free approach.  Lab on Chip 21 (2021) 2232-2243

      • Wong CFA, Vliet, L. D., Bhujbal SV, Guo, C., Sletmoen M., Stokke BT, Hollfelder, F., and Lale R. A titratable cell lysis-on-demand system for droplet-compartmentalized ultrahigh-throughput screening in functional metagenomics and directed evolution. ACS Synthetic Biology (2021, in press)

      Steven Ray Haakon Wilson

      Professor for Chemical Life Science and PI at the Hybrid Technology Hub - CoE, University of Oslo


      Keywords describing the research

      • mass spectrometry
      • separation science
      • automated sample preparation
      • small sample analysis
      • coupling with OoC

        Recent publications

        • Skottvoll, S. F., Hansen, F. A., Harrison, S., Boger, I. S., Mrsa, A., Restan, M. S., Stein, M., Lundanes, E., Pedersen-Bjergaard, S., Aizenshtadt, A., Krauss, S., Sullivan, G., Bogen, I. L, Wilson, S. R. Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies. Analytical chemistry 93 (7), 3576-3585 (2021)
        • Røberg-Larsen, H., Lundanes, E., Nyman, T.A., Berven, F.S., Wilson, S.R. Liquid chromatography, a key tool for the advancement of single-cell omics analysis. Analytica Chimica Acta, 338551 (2021).
        • Roberg-Larsen, H., Wilson, S.R., Lundanes, E. Recent advances in on-line upfront devices for sensitive bioanalytical nano LC methods. TrAC Trends in Analytical Chemistry, 116190 (2021).
        • Kogler, S., Harrison, S., Aizenshtadt, A., Skottvoll, F.S., Abadpour, S., Krauss, S., Scholz, H., Sullivan, G., Lundanes, E., Wilson, S.R. “Organoid-in-a-column” coupled on-line with liquid chromatography-mass spectrometry. bioRxiv (2020).

        • Harrison, S., Sillar, R., Tanaka, Y. et al. Scalable production of tissue-like vascularised liver organoids from human PSCs. bioRxiv (2020)

          Espen Melum

          Senior Researcher at Norwegian PSC research center, Oslo University Hospital


          Keywords describing the research

          • ile duct inflammation

          • immunology

          • unconventional t-cells

          • regenerative medicine

          • organoids

          • liver disease 

          Recent publications

          • Jiang X, Bergquist A, Löscher BS, Venkatesh G, Mold JE, Holm K, Laerdahl JK, Skånland SS, Maleki KT, Cornillet M, Taskén K, Franke A, Karlsen TH, Björkström NK, Melum E (2021) A heterozygous germline CD100 mutation in a family with primary sclerosing cholangitis.Sci Transl Med, 13 (582) DOI 10.1126/scitranslmed.abb0036, PubMed 33627483

          • Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Garcia-Bernardo J, Mahbubani K, Canu G, Gieseck R, Berntsen NL, Mulcahy VL, Crick K, Fear C, Robinson S, Swift L, Gambardella L, Bargehr J, Ortmann D, Brown SE, Osnato A, Murphy MP et al. (2021) Cholangiocyte organoids can repair bile ducts after transplantation in the human liver.
            Science, 371 (6531), 839-846
            DOI 10.1126/science.aaz6964, PubMed 33602855

          • Valestrand L, Berntsen NL, Zheng F, Schrumpf E, Hansen SH, Karlsen TH, Blumberg RS, Hov JR, Jiang X, Melum E (2020) Lipid antigens in bile from patients with chronic liver diseases activate natural killer T cells.
            Clin Exp Immunol, 203 (2), 304-314 DOI 10.1111/cei.13541, PubMed 33089489

          • Melum E, Jiang X, Baker KD, Macedo MF, Fritsch J, Dowds CM, Wang J, Pharo A, Kaser A, Tan C, Pereira CS, Kelly SL, Duan J, Karlsen TH, Exley MA, Schütze S, Zajonc DM, Merrill AH, Schuchman EH, Zeissig S, Blumberg RS (2019) Control of CD1d-restricted antigen presentation and inflammation by sphingomyelin. Nat Immunol, 20 (12), 1644-1655 DOI 10.1038/s41590-019-0504-0, PubMed 31636468

          • Berntsen NL, Fosby B, Tan C, Reims HM, Ogaard J, Jiang X, Schrumpf E, Valestrand L, Karlsen TH, Line PD, Blumberg RS, Melum E (2018)
            Natural killer T cells mediate inflammation in the bile ducts. Mucosal Immunol, 11 (6), 1582-1590
            DOI 10.1038/s41385-018-0066-8, PubMed 30115993

          Hanne Scholz

          Senior Researcher, Dep. of Transplantation, Oslo University Hospital.

          Vice-director of Hybrid Technology Hub - CoE, University of Oslo


          Keywords describing the research

          • human pancreatic islets
          • adult stem/stromal cells
          • regenerative medicine
          • cell therapy
          • pancreatic organoids
          • diabetes

            Recent publications

            • Abadpour, S., Wang, C., Niemi, E.M. et al. Tissue Engineering Strategies for Improving Beta Cell Transplantation Outcome. Curr Transpl Rep (2021). https://doi.org/10.1007/s40472-021-00333-
            • Ghila L, Legøy TA, Mathisen AF, Abadpour S, Paulo JA, Scholz H, Ræder H, Chera S. Chronically Elevated Exogenous Glucose Elicits Antipodal Effects on the Proteome Signature of Differentiating Human iPSC-Derived Pancreatic Progenitors. Int J Mol Sci. 2:22(7) (2021), 3698. 
            • Abadpour S, Tyrberg B, Schive SW, Huldt CW, Gennemark P, Ryberg E, Rydén-Bergsten T, Smith DM, Korsgren O, Skrtic S, Winzell MS*, Scholz H* (2020) Inhibition of the prostaglandin D2-GPR44/DP2 axis improves human islet survival and function. Diabetologia, 63 (7) (2020), 1355-1367.
            • Abadpour, S., A. Aizenshtadt, P. A. Olsen, K. Shoji, S. R. Wilson, S. Krauss, and H. Scholz. Pancreas-on-a-Chip Technology for Transplantation Applications. Curr Diab Rep 20, no. 12 (2020), 72.
            • Vethe H, Ghila L, Berle M, Hoareau L, Haaland A, Scholz H, Paulo JA, Chera S, Ræder H. The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation. Front Endocrinol (Lausanne), 10, 293 (2019). DOI 10.3389/fendo.2019.00293, PubMed 31139151
            • Schuetz C, Anazawa T, Cross SE, Labriola L, Meier RPH, Redfield RR, Scholz H, Stock PG, Zammit NW, IPITA YIC Young Investigator Committee β Cell Replacement Therapy: The Next 10 Years Transplantation. 102 (2) (2018), 215-229 DOI 10.1097/TP.0000000000001937, PubMed 28885496

              Research Groups in Sweden

              photographer: Malin Arnesson

              Caroline Beck Adiels

              Assoc. Prof. at the Biological Physics Lab, Department of Physics University of Gothenburg


              Keywords describing the research

              • Single-cell analysis

              • microfluidics

              • cell biology

              • optics

              • machine learning

              Recent publications

              • Intercellular communication induces glycolytic synchronization waves between individually oscillating cells. Martin Mojica Benavides, D. D. van Niekerk, M. Mijalkov, J. L. Snoep, Bernhard Mehlig, Giovanni Volpe, Mattias Goksör, Caroline B. Adiels Proceedings of the National Academy of Sciences of the United States of America - 2021-01-01

              • Fast and Accurate Nanoparticle Characterization Using Deep-Learning-Enhanced Off-Axis Holography. Benjamin Midtvedt, Erik Olsén, Fredrik Eklund, Fredrik Höök, Caroline B. Adiels, Giovanni Volpe, Daniel Midtvedt ACS Nano - 2021-01-01

              • Phosphofructokinase controls the acetaldehyde induced phase shift in isolated yeast glycolytic oscillators.

                David van Niekerk, Anna-Karin Gustavsson, Martin Mojica Benavides, Caroline B. Adiels, Mattias Goksör, Jacky L Snoep The Biochemical journal - 2019-01-01four

              • Design and fabrication of a scalable liver-lobule-on-a-chip microphysiological platform. Amin A Banaeiyan , J. Theobald, Jurgita Paukstyte, S. Wölfl, Caroline B. Adiels, Mattias Goksör Biofabrication 2017-01-01

              Volker Lauschke

              Assoc. Prof. in Personalized Medicine and Drug Development, Karolinska Institutet, Stockholm


              Keywords describing the research

              • drug development

              • translational research

              • precision medicine

              • tissue interactions

              • microphysiology

              Recent publications

              • Stebbing, J. and Lauschke, V.M. JAK inhibitors: more than just steroids. New England Journal of Medicine (In press).

              • Shen, J.X., Couchet, M., Dufau, J., de Castro Barbosa, T., Ulbrich, M., Helmstädter, M., Kemas, A.M., Zandi Shafagh, R., Marques, M.A., Hansen, J., Mejhert, N., Langin, D., Ryden, M., Lauschke, V.M. 3D adipose tissue culture links the organotypic microenvironment to improved adipogenesis. Advanced Science (In press).

              • Stebbing, J., Sanchez Nievas, G., Falcone, M., Youhanna, S., Richardson, P., Ottaviani, S., Shen, J.X., Sommerauer, C., Tiseo, G., Ghiadoni, L., Virdis, A., Monzani, F., Rizos, L.R., Forfori, F., Cespedes, A.A., De Marco, S., Carrozzi, L., Sanchez-Jurado, P.M., Bernardo, D.C., Mendez, L.S., Tan, Y.J., Monteil, V., Dranitsaris, G., Zhang, H., Pistello, M., Li, W., Romero, M.M., Pretel, A.F., Simon-Talero, R.S., Garcia-Molina, R., Kutter, C., Felce, J.H., Nizami, Z.F., Miklosi, A.G., Penninger, J.M., Menichetti, F., Mirazimi, A., Abizanda, P., Lauschke, V.M. Janus kinase inhibition reduces SARS-CoV-2 liver infectivity and modulates host cell inflammatory responses to reduce morbidity and mortality. Science Advances 2020 7(1):eabe4724. PMID 33187978.

              • Oliva-Vilarnau, N., Vorrink, S.U., Ingelman-Sundberg, M., Lauschke, V.M. A 3D cell culture model identifies Wnt/β-catenin mediated inhibition of p53 as a critical step during human hepatocyte regeneration. Advanced Science 2020 7(15):2000248. PMID 32775153.

              • Azzimato, V., Jager, J., Chen, P., Morgantini, C., Levi, L., Barreby, E., Sulen, A., Oses, C., Willerbrords, J., Xu, C., Li, X., Shen, J.X., Akbar, N., Haag, L., Ellis, E., Wålhen, K., Näslund, E., Thorell, A., Choudhury, R.P., Lauschke, V.M., Ryden, M., Craige, S.M., Aouadi, M. Liver macrophages inhibit the endogenous antioxidant response in obesity-associated insulin resistance. Science Translational Medicine 2020 12(532). PMID 32102936.

              Anna Herland

              Prof. at Herland Lab,

              KTH Royal Institute of Technology & at

              AIMES (Center for the Advancement of Integrated Medical and Engineering Sciences), Karolinska Institute, Stockholm


              Keywords describing the research

              • Central nervous system

              • Stem cells

              • neurovasculature

              • sensors

              • microfluidics

              Recent publications

              • Matthiesen, I., Voulgaris, D., Nikolakopoulou, P., Winkler, T. E. & Herland, A. (2021) Continuous monitoring reveals protective effects of N-acetylcysteine amide on an isogenic microphysiological model of the neurovascular unit. submitted and posted to bioRxiv(2021). doi:10.1101/2021.03.26.433307

              • Nikolakopoulou, P., Rauti, R., Voulgaris, D., Shlomy, I., Maoz, B. M., & Herland, A. (2020). Recent progress in translational engineered in vitro models of the central nervous system. Brain(2020). doi: 1093/brain/awaa268

              • Winkler, T. E., Feil, M., Stronkman, E. F. G. J., Matthiesen, I., & Herland, A. (2020). Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling. Lab on a Chip, 20(7), 1212–1226. doi:10.1039/D0LC00009D
              • Herland, A., Maoz, B. M., Das, D., Somayaji, M. R., Prantil-Baun, R., Novak, R., … Ingber, D. E. (2020). Quantitative prediction of human pharmacokinetic responses to drugs via fluidically coupled vascularized organ chips. Nature Biomedical Engineering (2020). doi:10.1038/s41551-019-0498-9.
              • *Maoz BM, Herland A*, FitzGerald EA, Grevesse T, Vidoudez C, Pacheco AR, Sheehy SP, Park T-E, Dauth S, Mannix R, Budnik N, Shores K, Cho A, Nawroth JC, Segrè D, Budnik B, Ingber DE, Parker KK. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic coupling of endothelial and neuronal cells. Nature Biotechnology. 2018;36:865-874. doi:10.1038/nbt.4226.

                Maria Tenje

                Prof. at Science for Life Laboratory (SciLifeLab), Division of Biomedical Engineering, Uppsala University, Uppsala


                Keywords describing the research

                • organs-on-chip

                • microfabrication

                • microfluidics

                • acoustofluidics

                Recent publications

                • Andersson, S. Johansson, H. Bergman, L. Xiao, L. Behrendt, M. Tenje. A microscopy-compatible temperature regulation system for single-cell phenotype analysis – demonstrated by thermoresponse mapping of microalgae, Lab on a Chip 21 (2021) 1694-1705
                • Raouf Atif, M. Pujari-Palmer, M. Tenje, G. Mestres. A microfluidics-based method for culturing osteoblasts on biomimetic hydroxyapatite, Acta Biomaterialia 127, (2021) 327-337
                • Kadekar, L. Barbe, M. Stoddart, O. P. Varghese, M. Tenje, G.Mestres. Effect of the Addition Frequency of 5-Azacytidine in Both Micro- and Macroscale Cultures, Cellular and Molecular Bioengineering 14 (2021) 121–130
                • Fornell, H. Pohlit, Q. Shi, M. Tenje. Acoustic focusing of beads and cells in hydrogel droplets, Scientific Reports 11 (2021) 7479
                • Paloschi, M. Sabater-Lleal, H. Middelkamp, A. Vivas, S. Johansson, A. van der Meer, M. Tenje, L. Maegdefessel. Organ-on-a-chip technology: a novel approach to investigate cardiovascular diseases, Cardiovascular Research (2021)