Keywords describing the research
High throughput couples organ systems
3D printing
intestine modelling
fluidics and dynamics
gelatin hydrogels
Recent publications
Tissue Engineering: 3D Printed Stackable Titer Plate Inserts Supporting Three Interconnected Tissue Models for Drug Transport Studies. (Adv. Biosys). 7/2020). Morten Leth Jepsen Andreas Willumsen Chiara Mazzoni Anja Boisen Line Hagner Nielsen Martin Dufva. 2020, Volume 4, Issue 7, pp. 1900289. https://doi.org/10.1002/adbi.201900289
Jepsen ML, Nielsen LH, Boisen A, Almdal K, Dufva M. Characterization of thin gelatin hydrogel membranes with balloon properties for dynamic tissue engineering. Biopolymers. 2019;110(1):e23241. doi:10.1002/bip.23241. https://doi.org/10.1002/bip.23241
Tan H-Y, Trier S, Rahbek UL, Dufva M, Kutter JP, Andresen T A multi-chamber microfluidic intestinal barrier model using Caco-2 cells for drug transport studies. PLoS. 2018;13(5):e0197101. https://doi.org/10.1371/journal.pone.0197101
Starokozhko V, Hemmingsen M, Larsen L, et al. Differentiation of human-induced pluripotent stem cell under flow conditions to mature hepatocytes for liver tissue engineering. J Tissue Eng Regen Med. 2018
Pimentel C R, Ko SK, Caviglia C, Wolff A, Emnéus J, Keller SS, Dufva M. Three-dimensional fabrication of thick and densely populated soft constructs with complex and actively perfused channel network. 2018. Acta Biomaterialia, 65, 174-184. https://doi.org/10.1016/j.actbio.2017.10.047
;12(5):1273-1284. https://doi.org/10.1002/term.2659
Keywords describing the research
Bio-Functional materials
Additive manufacturing
Instrumented Microphysiological Systems
Tissue engineering
Bio-printing
Recent publications
Keywords describing the research
brain organoids
conductive scaffolds
bioelectrochemistry
optogenetics
drug delivery
Recent publications
Keywords describing the research
cell fate
mechanobiology
cell organization and tissue remodeling
disease modeling
tissue engineering
Recent publications
Driessen RCH, Stassen OMJA, Sjöqvist M, Suarez Rodriguez F, Grolleman J, Bouten CVC, Sahlgren CM. Shear stress induces expression, intracellular reorganization and enhanced Notch activation potential of Jagged1. Integr Biol (Camb). 2018. https://doi.org/10.1039/c8ib00036k
Loerakker S*, Stassen OMJA, Ter Huurne FM, Boareto M, Bouten CVC, Sahlgren CM*. Mechanosensitivity of Jagged-Notch signaling can induce a switch-type behavior in vascular homeostasis. Proc Natl Acad Sci U S A. 2018. https://doi.org/10.1073/pnas.1715277115
Keywords describing the research
quantitative bioimaging
in-silico modelling
bioelectronics
biophysics
bioimpedance
Recent publications
Keywords describing the research
Recent publications
Cells, Volume 11, Issue 6, p. 1045. March 2022. DOI: 10.3390/cells11061045 https://www.mdpi.com/2073-4409/11/6/1045
Keywords describing the research
Recent publications
Bakreen, A, Juntunen, M, Dunlop, Y, Ugidos, IF, Malm, T, Miettinen, S et al.. Additive Behavioral Improvement after Combined Cell Therapy and Rehabilitation Despite Long-Term Microglia Presence in Stroke Rats. Int J Mol Sci. 2021;22 (4):. doi: 10.3390/ijms22041512. PubMed PMID:33546370 PubMed Central PMC7913568
Juntunen, M, Hagman, S, Moisan, A, Narkilahti, S, Miettinen, S. In Vitro Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons. Stem Cells Int. 2020;2020 :8841026. doi: 10.1155/2020/8841026. PubMed PMID:33178286 PubMed Central PMC7647751.
Keywords describing the research
Drug metabolism
drug-drug interactions
liver-on-a-chips
immobilized enzyme reactors
digital microfluidics
polymer microfabrication
Recent publications
Keywords describing the research
cancer research,
immunology
Recent publications
Alsaed B,…Saharinen P, Ilonen I, Haikala HM: Ex vivo modeling of precision immuno-oncology responses in lung cancer organ-on-a-chip. Under prep.
Ollila-Raj H, Murumägi A, Pellinen T, Arjama M, Sutinen E, Volmonen K, Haikala HM, Kallioniemi O, Mäyränpää MI, Ilonen I. Novel therapeutic approaches for pleural mesothelioma identified by functional ex vivo drug sensitivity testing. Lung Cancer. Apr;178:213-219, 2023
Haikala HM, Lopez T, Köhler J, Eser PO, Xu M, Zeng Q, Teceno TJ, Ngo K, Zhao Y, Ivanova EV, Bertram AA, Leeper BA, Chambers ES, Adeni AE, Taus LJ, Kuraguchi M, Kirschmeier PT, Yu C, Shiose Y, Kamai Y, Qiu Y, Paweletz CP, Gokhale PC, Jänne PA. EGFR Inhibition Enhances the Cellular Uptake and Antitumor-Activity of the HER3 Antibody-Drug Conjugate HER3-DXd. Cancer Res. Jan 1;82(1):130-141, 2022
Haikala HM, Jänne PA. Thirty Years of HER3: From Basic Biology to Therapeutic Interventions. Clin Cancer Res. 2021 Jul 1;27(13):3528-3539, 2021
Haikala HM, Anttila JM, Marques E, Raatikainen T, Ilander M, Hakanen H, Ala-Hongisto H, Savelius M, Balboa D, Von Eyss B, Eskelinen V, Munne P, Nieminen AI, Otonkoski T, Schüler J, Laajala TD, Aittokallio T, Sihto H, Mattson J, Heikkilä P, Leidenius M, Joensuu H, Mustjoki S, Kovanen P, Eilers M, Leverson JD, Klefström J. Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy. Nat Commun. Feb 6;10(1):620, 2019
Keywords describing the research
iPSC
cardiomyocytes
hepatocytes
disease modeling
Organ-on-Chip
Recent publications
Keywords describing the research
Recent publications
Keywords describing the research
organ-on-chip
in vitro models
disease modeling
micro-electrode arrays
neurons
astrocytes, microglia
Recent publications
Keywords describing the research
Breast
Lung
Morphogenesis
stem cells
drug screening
3D cell culture
Recent publications
Briem E, Budkova Z, Sigurdardottir AK, Hilmarsdottir B, Kricker J, Timp W, Magnusson MK, Traustadottir GA, Gudjonsson T. MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin. Mech Dev. 2019 Feb;155:34-47.
Arason AJ, Joelsson JP, Valdimarsdottir B, Sigurdsson S, Gudjonsson A, Halldorsson S, Johannsson F, Rolfsson O, Lehmann F, Ingthorsson S, Cherek P, Gudmundsson GH,
Gardarsson FR, Page CP, Baldursson O, Gudjonsson T, Kricker JA. Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia. Respir Res. 2019 Jun 24;20(1):129.
Morera E, Steinhäuser SS, Budkova Z, Ingthorsson S, Kricker J, Krueger A, Traustadottir GA, Gudjonsson T. YKL-40/CHI3L1 facilitates migration and invasion in HER2
overexpressing breast epithelial progenitor cells and generates a niche for capillary-like network formation. In Vitro Cell Dev Biol Anim. 2019 Dec;55(10):838-853.
Steinhaeuser SS, Morera E, Budkova Z, Schepsky A, Wang Q, Rolfsson O, Riedel A, Krueger A, Hilmarsdottir B, Maelandsmo GM, Valdimarsdottir B, Sigurdardottir AK,
Agnarsson BA, Jonasson JG, Ingthorsson S, Traustadottir GA, Oskarsson T, Gudjonsson T. ECM1 secreted by HER2-overexpressing breast cancer cells promotes formation of a vascular niche accelerating cancer cell migration and invasion. Lab Invest. 2020 Jul;100(7):928-944.
Schepsky, A., G. A. Traustadottir, J. P. Joelsson, S. Ingthorsson, J. Kricker, J. T. Bergthorsson, A. Asbjarnarson, T. Gudjonsson, N. Nupponen, A. Slipicevic, F. Lehmann and T. Gudjonsson (2020). Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines. Cancer Med 9(18): 6726-6738.
Keywords describing the research
Recent publications
Budkova, Z., Sigurdardottir, A.K., Briem, E., Bergthorsson, J.T., Sigurdsson, S., Magnusson, M.K., Traustadottir, G.A., Gudjonsson, T., and Hilmarsdottir, B. (2020). Expression of ncRNAs on the DLK1-DIO3 Locus Is Associated With Basal and Mesenchymal Phenotype in Breast Epithelial Progenitor Cells. Front Cell Dev Biol 8, 461.
Arason, A., Agnarsson, B.A., Johannesdottir, G., Johannsson, O.T., Hilmarsdottir, B., Reynisdottir, I., and Barkardottir, R.B. (2019). The BRCA1 c.4096+3A>G Variant Displays Classical Characteristics of Pathogenic BRCA1 Mutations in Hereditary Breast and Ovarian Cancers, But Still Allows Homozygous Viability. Genes (Basel) 10.
Grinde, M.T., Hilmarsdottir, B., Tunset, H.M., Henriksen, I.M., Kim, J., Haugen, M.H., Rye, M.B., Mælandsmo, G.M., and Moestue, S.A. (2019). Glutamine to proline conversion is associated with response to glutaminase inhibition in breast cancer. Breast Cancer Res. 21, 61.
Briem, E., Ingthorsson, S., Traustadottir, G.A., Hilmarsdottir, B., and Gudjonsson, T. (2019). Application of the D492 Cell Lines to Explore Breast Morphogenesis, EMT and Cancer Progression in 3D Culture. J Mammary Gland Biol Neoplasia.
Hilmarsdottir, B., Briem, E., Halldorsson, S., Kricker, J., Ingthorsson, S., Gustafsdottir, S., Mælandsmo, G.M., Magnusson, M.K., and Gudjonsson, T. (2017). Inhibition of PTP1B disrupts cell-cell adhesion and induces anoikis in breast epithelial cells. Cell Death Dis 8, e2769.
photographer: Malin Arnesson
Keywords describing the research
Developmental biology and morphogenetic signaling (HH/WNT)
Drug development on morphogenetic signals
Organ-on-a-chip platform development
Liver organoids Gastruloids
Recent publications
Thermoplastic elastomer (TPE) – Poly-(methyl methacrylate) (PMMA) hybrid devices for active pumping PDMS-free Organ-on-a-Chip systems. Biosensors 2021, 11(5), 162. Mathias Busek, Steffen Nøvik, Aleksandra Aizenshtadt, Mikel Amirola-Martinez, Thomas Combriat, Stefan Grünzner, Stefan Krauss
Pre-clinical lead optimization of a 1,2,4-triazole based tankyrase inhibitor. J Med Chem. 2020, Jul 9;63(13):6834-6846. Jo Waaler, Ruben G. G. Leenders, Sven T. Sowa, Shoshy Alam Brinch, Max Lycke, Piotr Nieczypor, Sjoerd Aertssen, Sudarshan Murthy, Albert Galera-Prat, Eddy Damen, Anita Wegert, Marc Nazaré, Lari Lehtiö, and Stefan Krauss
3D cell culture models and organ‐on‐a‐chip: Meet separation science and mass spectrometry. Electrophoresis 2020, 41 (1-2), 56-64. Ann Lin, Frøydis Sved Skottvoll, Simon Rayner, Stig Pedersen‐Bjergaard, Gareth Sullivan, Stefan Krauss, Steven Ray Wilson, Sean Harrison
Pancreas-on-a-chip technology for transplantation applications. Current Diabetes Reports 2020, 20 (12), 1-13. Shadab Abadpour; Aleksandra Aizenshtadt; Petter Angell Olsen; Kayoko Shoji; Steven Ray Wilson; Stefan Krauss; Hanne Scholz
The tankyrase inhibitor G007-LK inhibits small intestine Lgr5+ stem cell proliferation without altering tissue morphology. Biol Res. 2018, Jan 9;51(1):3. Jens Henrik Norum, Ellen Skarpen, Andreas Brech, Raoul Kuiper, Wanja Kildal, Jo Waaler, Stefan Krauss and Therese Sørlie
Keywords describing the research
Biopolymers
Single-molecule
Hydrogels
Biosensing
Microfluidics
Recent publications
Håti, A.G., Basset, D., Ribe, J.M., Sikorski, P., Weitz, D.A., and Stokke, B.T. Versatile, cell and chip friendly method to gel alginate in microfluidic devices. Lab on a Chip 16 (2016) 3718-3727.
Jonasova, E.P., Bjørkøy, A., and Stokke, B.T., Toehold length of target ssDNA affects its reaction-diffusion behaviour in DNA-responsive DNA-co-acrylamide hydrogels; Biomacromolecules, 21 (2020) 1687-1699
Øvreeide, I.H., Zoellner, A., Mielnik, M.M. and Stokke, B.T. Curved passive mixing structures: A robust design to obtain efficient mixing and mass transfer in microfluidic channels. J. Micromech. Microeng. 31 (2021) 015006
Ahmed, H., and Stokke, B.T., Fabrication of monodisperse alginate microgel beads by microfluidic picoinjection: A chelate free approach. Lab on Chip 21 (2021) 2232-2243
Wong CFA, Vliet, L. D., Bhujbal SV, Guo, C., Sletmoen M., Stokke BT, Hollfelder, F., and Lale R. A titratable cell lysis-on-demand system for droplet-compartmentalized ultrahigh-throughput screening in functional metagenomics and directed evolution. ACS Synthetic Biology (2021, in press)
Keywords describing the research
Recent publications
Kogler, S., Harrison, S., Aizenshtadt, A., Skottvoll, F.S., Abadpour, S., Krauss, S., Scholz, H., Sullivan, G., Lundanes, E., Wilson, S.R. “Organoid-in-a-column” coupled on-line with liquid chromatography-mass spectrometry. bioRxiv (2020).
Harrison, S., Sillar, R., Tanaka, Y. et al. Scalable production of tissue-like vascularised liver organoids from human PSCs. bioRxiv (2020)
Keywords describing the research
ile duct inflammation
immunology
unconventional t-cells
regenerative medicine
organoids
liver disease
Recent publications
Jiang X, Bergquist A, Löscher BS, Venkatesh G, Mold JE, Holm K, Laerdahl JK, Skånland SS, Maleki KT, Cornillet M, Taskén K, Franke A, Karlsen TH, Björkström NK, Melum E (2021) A heterozygous germline CD100 mutation in a family with primary sclerosing cholangitis.Sci Transl Med, 13 (582) DOI 10.1126/scitranslmed.abb0036, PubMed 33627483
Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Garcia-Bernardo J, Mahbubani K, Canu G, Gieseck R, Berntsen NL, Mulcahy VL, Crick K, Fear C, Robinson S, Swift L, Gambardella L, Bargehr J, Ortmann D, Brown SE, Osnato A, Murphy MP et al. (2021) Cholangiocyte organoids can repair bile ducts after transplantation in the human liver.
Science, 371 (6531), 839-846
DOI 10.1126/science.aaz6964, PubMed 33602855
Valestrand L, Berntsen NL, Zheng F, Schrumpf E, Hansen SH, Karlsen TH, Blumberg RS, Hov JR, Jiang X, Melum E (2020) Lipid antigens in bile from patients with chronic liver diseases activate natural killer T cells.
Clin Exp Immunol, 203 (2), 304-314 DOI 10.1111/cei.13541, PubMed 33089489
Melum E, Jiang X, Baker KD, Macedo MF, Fritsch J, Dowds CM, Wang J, Pharo A, Kaser A, Tan C, Pereira CS, Kelly SL, Duan J, Karlsen TH, Exley MA, Schütze S, Zajonc DM, Merrill AH, Schuchman EH, Zeissig S, Blumberg RS (2019) Control of CD1d-restricted antigen presentation and inflammation by sphingomyelin. Nat Immunol, 20 (12), 1644-1655 DOI 10.1038/s41590-019-0504-0, PubMed 31636468
Berntsen NL, Fosby B, Tan C, Reims HM, Ogaard J, Jiang X, Schrumpf E, Valestrand L, Karlsen TH, Line PD, Blumberg RS, Melum E (2018)
Natural killer T cells mediate inflammation in the bile ducts. Mucosal Immunol, 11 (6), 1582-1590
DOI 10.1038/s41385-018-0066-8, PubMed 30115993
Keywords describing the research
Recent publications
photographer: Malin Arnesson
Keywords describing the research
Single-cell analysis
microfluidics
cell biology
optics
machine learning
Recent publications
Intercellular communication induces glycolytic synchronization waves between individually oscillating cells. Martin Mojica Benavides, D. D. van Niekerk, M. Mijalkov, J. L. Snoep, Bernhard Mehlig, Giovanni Volpe, Mattias Goksör, Caroline B. Adiels Proceedings of the National Academy of Sciences of the United States of America - 2021-01-01
Fast and Accurate Nanoparticle Characterization Using Deep-Learning-Enhanced Off-Axis Holography. Benjamin Midtvedt, Erik Olsén, Fredrik Eklund, Fredrik Höök, Caroline B. Adiels, Giovanni Volpe, Daniel Midtvedt ACS Nano - 2021-01-01
Phosphofructokinase controls the acetaldehyde induced phase shift in isolated yeast glycolytic oscillators.
David van Niekerk, Anna-Karin Gustavsson, Martin Mojica Benavides, Caroline B. Adiels, Mattias Goksör, Jacky L Snoep The Biochemical journal - 2019-01-01four
Design and fabrication of a scalable liver-lobule-on-a-chip microphysiological platform. Amin A Banaeiyan , J. Theobald, Jurgita Paukstyte, S. Wölfl, Caroline B. Adiels, Mattias Goksör Biofabrication 2017-01-01
Keywords describing the research
drug development
translational research
precision medicine
tissue interactions
microphysiology
Recent publications
Keywords describing the research
Central nervous system
Stem cells
neurovasculature
sensors
microfluidics
Recent publications
Matthiesen, I., Voulgaris, D., Nikolakopoulou, P., Winkler, T. E. & Herland, A. (2021) Continuous monitoring reveals protective effects of N-acetylcysteine amide on an isogenic microphysiological model of the neurovascular unit. submitted and posted to bioRxiv(2021). doi:10.1101/2021.03.26.433307
Nikolakopoulou, P., Rauti, R., Voulgaris, D., Shlomy, I., Maoz, B. M., & Herland, A. (2020). Recent progress in translational engineered in vitro models of the central nervous system. Brain(2020). doi: 1093/brain/awaa268
Keywords describing the research
organs-on-chip
microfabrication
microfluidics
acoustofluidics
Recent publications